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Supplemental Figure 7: Knockdown of P-TEFb components CDK9.S and CYCLINT1 does not affect Snail1 expression.
All embryos were injected in one cell of a two cell stage embryo with either a control morpholino CoMo, 40ng CDK9a morpholino or 60ng of CyclinT1 morpholino. 300pg of Lac-Z cRNA was co-injected with morpholinos and β-gal activity was detected using an X-gal stain to monitor lineage tracing. in situ hybridisation was then carried out with a Snail1 probe. Note the Snail 1 expression on the injected side with the Cdk9 and CyclineT1 morpholinos looks unchanged or very slightly increased. Embryos are shown as dorsal views with anterior to the top. |
