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Fig. 1. Migration of neural crest is required for expression of noradrenergic markers in the anteroventral region
Xenopus embryos were injected in one of the two-cell stage with beta-galactosidase (light blue) as a tracer along with either GFP as a control, or mRNA encoding a dominant negative form of Slug (dnSlug) to inhibit neural crest migration, injected side to the right. (A) Embryos were fixed at stage 17/18 and subject to in situ hybridization for the neural crest markers Slug, Snail, and FoxD3 and noradrenergic markers Phox2a and Hand2. (B) Embryos were scored compared to the uninjected side based on inhibition of migration for neural crest markers and reduced expression of noradrenergic markers (See Supplementary Fig.2). For scoring data, [n=28-45]. Kruskal-Wallis non-parametric ANOVA was performed comparing GFP control with dnSlug-injected embryos (p-values of <0.05 = *, <0.0005 = **, and <0.000005 = ***). |
