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Figure 2. Tet3 Directly Regulates Key Developmental Genes. (A) Expression level changes of developmental genes resulting from Tet3 depletion as shown by in situ hybridization at stage 14. Red arrows show the inhibited expression by xlTet3 MOs injection, whereas black arrows show rescued expression by xtTet3 coinjection. L, left; R, right; a, anterior; p, posterior; d, dorsal; v, ventral. The red dots are injection tracer by β-gal staining.(B) RT-qPCR confirms the differentially expressed genes after Tet3 depletion at stage 14. Control MO, xlTet3 MOs or xlTet3 MOs/xtTet3 was injected into two dorsal blastomeres at 4-cell stage. Relative gene expression was normalized to odc. Results are shown as mean ± SD (n = 3).(C) Tet3 occupancy at target gene promoters by ChIP-qPCR assay. Data are presented as mean ± SEM (n = 3).See also Figure S2. |
