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Figure 7. A-E prime : X-Delta-2 loss of function affects cranial neural crest cell migration. Noninjected (Nic) controls (A-E) and embryos injected on the left-hand side with X-Delta-2 morpholino (MO; A prime -E prime ) were analyzed for the expression of cranial neural crest markers or stained with Alcian blue to highlight the cartilage. A,A prime ,B,B prime show stage 21 embryos from the anterior side with dorsal to the top; C,C prime ,D,D prime show stage 25 embryos from the lateral side with dorsal to the top; and in E,E prime show stage 48 embryos from the ventral side with anterior to the top. Cranial neural crest cells fail to migrate as shown by the expression of X-Slug (A prime ), Xsnail (B prime ), and X-dll4 (C prime ,D prime ). The cartilage staining shows that the gills, derived from the branchial arches, do not form in the X-Delta-2 MO-injected side (arrow in E prime ). The asterisk indicates the injected side. Nic for X-dll4 in C and D is the noninjected side of the same embryos shown in C prime ,D prime , respectively. |
