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Fig. S3. Temporal expression of PSC genes. (A-DD) Shown is the expression of foxi1 (A-E), atp6v0a4 (F-J), pendrin (K-O), pendrin-like (P-T), ae1 (U-Y) and ubp1 (Z-DD) across different stages of embryonic development. Embryonic stage (Nieuwkoop and Faber, 1967) is marked in the lower left corner. Note that expression of foxi1 precedes any other PSC marker (compare A with F,K,P,U,Z), that expression of atp6v0a4, pendrin, pendrin-like and ubp1 are all roughly simultaneous (F,K,P,Z), and that ae1 expression does not initiate until several stages later (U). Number of cells expressing pendrin, pendrin-like or ae1 is a subset of all PSCs (for example, compare I with N,T,X; see also Figs 4 and 5 in the main text). Finally, note the ae1 expression in ventral blood islands (arrowhead in Y); a splice variant of mammalian ae1 is strongly expressed in red blood cells (Kopito and Lodish, 1985).
References
Kopito, R. R. and Lodish, H. F. (1985). Primary structure and transmembrane orientation of the murine anion exchange protein. Nature 316, 234-238.
Nieuwkoop, P. D. and Faber, J. (1967). Normal Table of Xenopus Laevis, Amsterdam: North Holland. |
