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Figure 8. Depletion of FoxA4 led to head defects at tailbud stages.
Anterior views of stage 27/28 embryos injected with 20 ng of CtrlMO (A–E) or FoxA4MO (G–K). Embryos were injected into one dorsal cell at 4-cell stage (A, G) or prior to the first cleavage (B–E, H–K). Expression of otx2 (A, B, G, H), six3 (C, I), emx1 (D, J) and Xanf1 (E, K). Results are summarised in F, L. In embryos unilaterally injected with FoxA4MO, the telencephalic and caudal diencephalic otx2 subdomains were fused in the injected side (red arrowhead), and the eye was lost (70%, n = 24) (G). When FoxA4MO was homogenously distributed, the otx2 domain was reduced, the telencephalic subdomain was fused in the midline and also, with the caudal diencephalic subdomain (80%, n = 20) (H). FoxA4MO reduced the expression domains of six3 (87%, n = 23) (I), emx1 (80%, n = 26) (J) and Xanf1 (70%, n = 20) (K). dtel, dorsal telencephalon; vtel, ventral telencephalic otx2; cdi, caudal diencephalic otx2; mes, mesencephalic otx2; op, olfactory placode; epiph, epiphisis; ev, eye vesicle; cg, cement gland; pit, pituitary anlage; is, injected side; nis, non-injected side. The black line in F, L represents the contour of the head. For comparison, the head contour of the CtrlMO-injected embryo was projected on the FoxA4 morphant diagram (dotted black line in L).
doi:10.1371/journal.pone.0110559.g008 |
