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Fig. S7. An enlarged eye phenotype begins to appear after stage 28. (A-O) Xenopus embryos injected with synthetic RNAs as indicated on the left side (A, B, E, F, I, J and C, D, G, H, K, L) were subjected to in situ hybridization with antisense RNA probes including rax (A-D), pax6 (E-H) and six3 (I-L). Injected side (A, C, E, G, I and K). Uninjected side (B, D, F, H, J and L). (M-O) Percentages of embryos that an eye region of the injected side is enlarged compared to uninjected side. Eye regions are delineated by the expression of rax (M), pax6 (N) and six3 (O). (lacZ: stage 28, n = 17 (M), n = 18 (N), n = 14 (O); stage 32/33, n = 18 (M), n = 22 (N), n = 20 (O); stage 35/36, n = 16 (M), n = 17 (N), n = 18 (O). lacZ/ubc9/hmgb3: stage 28, n = 30 (M), n = 22 (N), n = 29 (O); stage 32/33, n = 28 (M), n = 31 (N), n = 38 (O); stage 35/36, n = 24 (M), n = 23 (N), n = 26 (O)) |
