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Figure S2. The Regulation of Gene Expression by Tet3, Related to Figure 2. (A) The inhibition of pax6, rx and six3 expression by Tet3 depletion in stage 19 embryos (anterior view). Red arrows indicate the inhibited expression. Red dots are injection tracer by b-gal staining. (B and C) Depletion of Tet3 by xlTet3 MOs injection does not cause significant changes in the expression of otx2 or sox2 genes at stage 14 by in situ hybridization (B) or RT-qPCR (C). L: left; R: right; d: dorsal; v: ventral; a: anterior; p: posterior. Red dots are injection tracer by b-gal staining. In (C), relative expressions were normalized to odc, and results are shown as mean SD (n = 3). (D) The summarized results of four independent phenotype rescue experiments. The phenotype was examined at stage 35. Partial defect means mildly abnormal head structure, small eyes or one eye; Complete defect means abnormal head structure and no eyes. |
