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Figure 2. Admp Expression Is Triggered by Low BMP Signaling, and Admp MO Dorsalizes the Embryo. (A) The Xenopus ventral and dorsal centers. (B) Admp is expressed dorsally (stage 12). (C) Chordin protein injection in the blastula cavity (5 ng) leads to upregulation of Admp expression. (D) Admp MO targeting both Xenopus laevis Admp pseudoalleles. (E) Xenopus Admp translation is specifically inhibited by Admp MO but not by control MO of random sequence. (F) ADMP causes phosphorylation of endogenous Smad1 in Xenopus embryos, which is blocked by Chordin protein injection.(G and H) Admp MO-injected embryos (12 ng total) are dorsalized.(I) zAdmp mRNA (75 pg total) ventralizes the Xenopus embryo.(J) Admp MO rescued by zAdmp mRNA. |
