|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fgf8
|
|
xenopus
|
NF stage 17
|
anterior neural fold
|
fgf8
|
|
xenopus
|
NF stage 17
to
NF stage 18
|
midbrain-hindbrain boundary
,
neuroectoderm
,
anterior neural fold
,
neural tube
|
pax2
|
|
xenopus
|
NF stage 33 and 34
|
retina
,
ventral
|
rax
|
|
xenopus
|
NF stage 14
|
eye primordium
,
optic vesicle
|
rax
|
|
xenopus
|
NF stage 17
|
retina
,
eye primordium
,
optic vesicle
|
vax2
|
|
xenopus
|
NF stage 33 and 34
|
retina
,
ventral
,
optic stalk
|
aldh1a3
|
|
xenopus
|
NF stage 10.25
|
blastopore lip
,
upper blastopore lip
|
aldh1a3
|
|
xenopus
|
NF stage 11
|
dorsal
|
aldh1a3
|
|
xenopus
|
NF stage 23
|
midbrain-hindbrain boundary
,
otic placode
,
optic vesicle
,
dorsal
,
ventral
|
aldh1a3
|
|
xenopus
|
NF stage 33 and 34
|
olfactory placode
,
retina
,
optic vesicle
,
ventral
|
aldh1a3
|
|
xenopus
|
NF stage 59
to
NF stage 60
|
retina
,
eye
,
ventral
|
aldh1a3
|
|
xenopus
|
NF stage 12.5
|
chordal neural plate
,
dorsal
|
aldh1a3
|
|
xenopus
|
NF stage 31
|
olfactory placode
,
otic vesicle
,
retina
,
midbrain-hindbrain boundary
,
cement gland
,
[+]
|
aldh1a2
|
|
xenopus
|
NF stage 17
|
eye primordium
,
optic vesicle
,
anterior neural fold
|
|
|
Fig. 4. Expression pattern of Raldh3 during Xenopus development. (A) Raldh3 expression as detected by whole-mount in situ hybridization of whole embryos (stage 10.25-31) or dissected neural retinas, without lens and pigmented epithelium (stage 59). cf, choroid fissure. Scale bar: 350 μm. (B) Raldh3 expression in transverse sections of stage 33 embryos after whole-mount in situ hybridization. From left to right, sections show expression at progressively posterior levels. op, olfactory placode; os, optic stalk. (C) Comparison of Pax2, Raldh3 and Vax2 expression in the optic cup in transversal sections of stage 33 embryos after whole-mount in situ hybridization. (D) Double in situ hybridizations of Rx1 with Raldh2, Shh or FGF8 on mid- to late neurula embryos shown from anterior view. The inset shows a double in situ hybridization of Rx1 with FGF8 on an early neurula embryo. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
fgf8
|
|
xenopus
|
NF stage 18
|
ectoderm
,
midbrain-hindbrain boundary
,
neuroectoderm
,
anterior neural fold
,
lateral plate mesoderm
|
fgf8
|
|
xenopus
|
NF stage 33 and 34
|
olfactory placode
,
otic vesicle
,
retina
,
midbrain-hindbrain boundary
,
pharyngeal arch
,
[+]
|
aldh1a2
|
|
xenopus
|
NF stage 18
|
retina
,
optic vesicle
,
ventral
,
anterior neural fold
|
aldh1a2
|
|
xenopus
|
NF stage 33 and 34
|
otic vesicle
,
retina
,
dorsal
,
ventral
,
lateral line placode
,
[+]
|
|
|
Fig. 9. RA, Hh and FGFR signaling pathways can crossregulate each other at the transcriptional level. (A) High dose RA treatments (10 μM) strongly repress Shh expression in the anterior midline (indicated by the yellow bracket) both at neurula and stage 33. The same RA doses cause fusion of the two stripes of FGF8 expression in the ANR and the anteroventral ectoderm in neurula stage embryos, while at stage 33 FGF8 expression is strongly downregulated in the whole head region. Low dose RA treatments (0.1 μM) have weaker effects on both Shh and FGF8. (B) Unilateral injection of 500 pg bhh mRNA laterally expands FGF8 expression in the anterior neural ridge but does not affect Raldh2 expression at neurula stages. The inset shows FGF8 expression in the ANR of an uninjected embryo at the same stage. The same dose of bhh upregulates both FGF8 and Raldh2 in the eye region at stage 33. (C) Unilateral injection of 4 pg iFGFR1 mRNA, followed by induction with AP20187 at stage 12.5/13, expands Shh expression in the prospective hypothalamic region at neurula, and activates Shh in the eye regions at stage 33. At doses of 4 and 2 pg, iFGFR-1 mRNA has no significant effect on Raldh2 expression at neurula stages and stage 33, respectively. |