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Experiment details for shh

A Retinoic Acid-Hedgehog Cascade Coordinates Mesoderm-Inducing Signals and Endoderm Competence during Lung Specification.

A Retinoic Acid-Hedgehog Cascade Coordinates Mesoderm-Inducing Signals and Endoderm Competence during Lung Specification.

Gene Clone Species Stages Anatomy
shh.L laevis NF stage 20 notochord
shh.L laevis NF stage 23 to NF stage 25 head region , notochord , foregut
shh.L laevis NF stage 29 and 30 to NF stage 32 brain , head region , foregut , lung primordium , neural tube , [+]
shh.L laevis NF stage 33 and 34 to NF stage 35 and 36 foregut , lung primordium , neural tube , gall bladder primordium , pharyngeal region

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  Figure S2 A, relevant to Figure 2. Additional analyses of the Hh/Gli pathway function in Xenopus. (A) In situ hybridization of Hh pathway components during different stages of Xenopus development prior to and during lung induction. The foregut / presumptive lung region (boxed) and sections through the lung field (dashed line) are shown at NF 34/35. Robust expression of the HH target genes ptch1 and gli1 in the foregut region is not detectable until NF30-32, coincident with the timing of shh induction in the foregut region. Smo, gli2, and gli3 are expressed in foregut mesoderm but not endoderm. Wnt2b is robustly detected at NF 34/35 but not at earlier stages.

Gene Clone Species Stages Anatomy
shh.L laevis NF stage 35 and 36 head region , endoderm , foregut , neural tube , pharyngeal region

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  Figure 1. Bidirectional Endoderm-Mesoderm Signaling Is Required for Respiratory Specification in the Xenopus Foregut. (A) Model of mesoderm-endoderm paracrine signaling during foregut organogenesis. (B) Schematic of the Xenopus organ induction assay. The foregut endoderm (yellow) and mesoderm (orange) were isolated at NF20 and cultured together or separately until NF36–38. (C) In situ hybridization using the indicated probes reveals that induction of nkx2-1/sftpc+ respiratory progenitors requires the mesoderm, whereas wnt2, wnt2b, and gli1 expression in the mesoderm requires the endoderm (outlines in yellow dashed line in embryo sections). The number of explants expressing the marker is indicated in the lower right of each image. See also Figure S1.