|
Gene |
Clone |
Species |
Stages |
Anatomy |
shh.L
|
|
laevis
|
NF stage 20
|
notochord
|
shh.L
|
|
laevis
|
NF stage 23
to
NF stage 25
|
head region
,
notochord
,
foregut
|
shh.L
|
|
laevis
|
NF stage 29 and 30
to
NF stage 32
|
brain
,
head region
,
foregut
,
lung primordium
,
neural tube
,
[+]
|
shh.L
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
foregut
,
lung primordium
,
neural tube
,
gall bladder primordium
,
pharyngeal region
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gli2
|
|
laevis
|
NF stage 20
|
optic vesicle
,
paraxial mesoderm
,
neural tube
|
gli2
|
|
laevis
|
NF stage 23
to
NF stage 25
|
head mesoderm
,
presomitic mesoderm
,
optic vesicle
,
paraxial mesoderm
,
neural tube
|
gli2
|
|
laevis
|
NF stage 29 and 30
to
NF stage 32
|
brain
,
spinal cord
,
mesoderm
,
head mesoderm
,
eye
,
[+]
|
gli2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
brain
,
spinal cord
,
mesoderm
,
pharyngeal arch
,
eye
,
[+]
|
gli1
|
|
laevis
|
NF stage 20
|
head region
,
paraxial mesoderm
|
gli1
|
|
laevis
|
NF stage 23
to
NF stage 25
|
head region
,
presomitic mesoderm
,
paraxial mesoderm
|
gli1
|
|
laevis
|
NF stage 29 and 30
to
NF stage 32
|
head region
,
somite
,
pharyngeal region
|
gli1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
head region
,
lateral plate mesoderm
,
pharyngeal region
|
dhh
|
|
laevis
|
NF stage 20
|
endoderm
,
foregut
|
dhh
|
|
laevis
|
NF stage 23
to
NF stage 25
|
endoderm
,
hindgut
,
foregut
|
dhh
|
|
laevis
|
NF stage 29 and 30
to
NF stage 32
|
endoderm
,
hindgut
,
foregut
|
dhh
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
head region
,
endoderm
,
hindgut
,
foregut
|
gli3
|
|
laevis
|
NF stage 20
|
paraxial mesoderm
,
neural tube
|
gli3
|
|
laevis
|
NF stage 23
to
NF stage 25
|
brain
,
mesoderm
,
somite
,
tail bud
,
foregut
,
[+]
|
gli3
|
|
laevis
|
NF stage 29 and 30
to
NF stage 32
|
head region
,
mesoderm
,
somite
,
foregut
,
pharyngeal region
|
gli3
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
spinal cord
,
head region
,
mesoderm
,
somite
,
foregut
,
[+]
|
smo
|
|
laevis
|
NF stage 20
|
mandibular crest
,
hyoid crest
,
branchial crest
,
presomitic mesoderm
,
optic vesicle
,
[+]
|
smo
|
|
laevis
|
NF stage 23
to
NF stage 25
|
presomitic mesoderm
,
optic vesicle
,
paraxial mesoderm
,
cranial neural crest
|
smo
|
|
laevis
|
NF stage 29 and 30
to
NF stage 32
|
hindbrain
,
head region
,
pronephric mesenchyme
,
lateral plate mesoderm
,
pharyngeal region
|
smo
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
head region
,
pronephric duct
,
lateral plate mesoderm
,
pronephric kidney
,
pharyngeal region
|
ptch1
|
|
laevis
|
NF stage 20
|
paraxial mesoderm
,
lateral plate mesoderm
|
ptch1
|
|
laevis
|
NF stage 23
to
NF stage 25
|
presomitic mesoderm
,
paraxial mesoderm
,
lateral plate mesoderm
|
ptch1
|
|
laevis
|
NF stage 29 and 30
to
NF stage 32
|
head region
,
somite
,
lateral plate mesoderm
,
pharyngeal region
|
ptch1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
head region
,
somite
,
lateral plate mesoderm
,
pharyngeal region
|
wnt2b
|
|
laevis
|
NF stage 20
|
anterior neural tube
|
wnt2b
|
|
laevis
|
NF stage 23
to
NF stage 25
|
anterior neural tube
|
wnt2b
|
|
laevis
|
NF stage 29 and 30
to
NF stage 32
|
midbrain
|
wnt2b
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
otic vesicle
,
midbrain
,
mesoderm
,
foregut
,
lens placode
|
|
|
Figure S2 A, relevant to Figure 2. Additional analyses of the Hh/Gli pathway function in Xenopus. (A) In situ hybridization of Hh pathway components during different stages of Xenopus development prior to and during lung induction. The foregut / presumptive lung region (boxed) and sections through the lung field (dashed line) are shown at NF 34/35. Robust expression of the HH target genes ptch1 and gli1 in the foregut region is not detectable until NF30-32, coincident with the timing of shh induction in the foregut region. Smo, gli2, and gli3 are expressed in foregut mesoderm but not endoderm. Wnt2b is robustly detected at NF 34/35 but not at earlier stages. |
|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gli2
|
|
laevis
|
NF stage 35 and 36
|
head region
,
splanchnic layer of lateral plate mesoderm
,
pronephric kidney
,
neural tube
,
pharyngeal region
|
gli1
|
|
laevis
|
NF stage 35 and 36
|
head region
,
splanchnic layer of lateral plate mesoderm
,
neural tube
,
pharyngeal region
|
bmp4
|
|
laevis
|
NF stage 35 and 36
|
head region
,
splanchnic layer of lateral plate mesoderm
,
pharyngeal region
|
gli3
|
|
laevis
|
NF stage 35 and 36
|
head region
,
splanchnic layer of lateral plate mesoderm
,
pronephric kidney
,
neural tube
,
pharyngeal region
|
nkx2-1
|
|
laevis
|
NF stage 35 and 36
|
forebrain
,
endoderm
,
foregut
,
thyroid primordium
,
lung primordium
|
wnt2
|
|
laevis
|
NF stage 35 and 36
|
midbrain-hindbrain boundary
,
head region
,
splanchnic layer of lateral plate mesoderm
|
sftpc
|
|
laevis
|
NF stage 35 and 36
|
endoderm
,
foregut
,
lung primordium
|
wnt2b
|
|
laevis
|
NF stage 35 and 36
|
lens
,
midbrain-hindbrain boundary
,
splanchnic layer of lateral plate mesoderm
|
|
|
Figure 1. Bidirectional Endoderm-Mesoderm Signaling Is Required for Respiratory Specification in the Xenopus Foregut.
(A) Model of mesoderm-endoderm paracrine signaling during foregut organogenesis.
(B) Schematic of the Xenopus organ induction assay. The foregut endoderm (yellow) and mesoderm (orange) were isolated at NF20 and cultured together or separately until NF36–38.
(C) In situ hybridization using the indicated probes reveals that induction of nkx2-1/sftpc+ respiratory progenitors requires the mesoderm, whereas wnt2, wnt2b, and gli1 expression in the mesoderm requires the endoderm (outlines in yellow dashed line in embryo sections). The number of explants expressing the marker is indicated in the lower right of each image.
See also Figure S1. |