| |
Figure 9. In situ hybridization localized Xhh mRNA in the notochord and floor
plate. In situ hybridization was performed on stage 32 X.1aevis embryos with
a digoxygenin labeled Xhh cRNA and the expression of Xhh mRNA was
analyzed after cross-sectioning the hybridized embryos. The most intense
hybridization was detected in the notochord (N) and the neural floor plate (FP).
The background signal below the notochord is likely due to non-specific
binding by the yolk proteins present in early embryos. |
