| |
Fig. 6. Expression of early genes in the chordoneural hinge. (A) Sagittal
section of a stage 32 tail bud whole mount in situ hybridisation to show
expression of Xnot2 (dark staining). Transcripts are restricted to the chordoneural
hinge and posterior ventral neural tube as previously reported
(Gont et al., 1993). (B) schematic diagram of Xnot2 expression based on
(A). (C) Sagittal section of stage 30 tail bud to show Xlim1 expression in
the chordoneural hinge and posterior notochord. There is also punctate
expression of Xlim1 in the neural tube more anteriorly. Xlim1 transcripts
are clearly absent from the tip of the chordoneural hinge in the sagittal
section, suggesting that expression is excluded from the M region. (D)
Schematic diagram of Xlim1 expression in the tail bud based on (C). (E)
Sagittal section of stage 30 tail bud to show Xshh expression in the chordoneural
hinge, notochord and floorplate of the neural tube and underlying
endoderm. (F) Schematic diagram of Xshh expression based on (E). Scale
bar in (A,C,E), 250 mm. Abbreviations: cnh, chordoneural hinge; end,
endoderm; nc, notochord; nt, neural tube; pw, posterior wall. |
