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sftpcxenopus   

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Experiment details for sftpc

Rankin SA et al. (2014) Assay

A Molecular atlas of Xenopus respiratory system development.

Gene Clone Species Stages Anatomy
sftpc.S laevis NF stage 33 and 34 to NF stage 35 and 36 endoderm , foregut , lung primordium

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  Fig. 10. Functional epistasis of Wnt/b-catenin, FGF, and BMP pathways. A–O: Wnt/b-catenin is necessary and sufficient downstream of FGF to induce respiratory fate. Embryos were injected at the 4-cell stage in both dorsal anterior blastomeres with control-MOs or wnt2/2b-MOs (10 ng/ blastomere) and then injected at the 8-cell stage dorsal-vegetally with 800 pg iFGFR1 mRNA (400 pg/blastomere). Subsequently embryos were treated from stages NF25–NF35 with either, DMSO, PD173074 (100 mM), DMH1 (20 mM), or AP20187 (2 mM to induce the iFGFR1 construct activ- ity). Embryos were analyzed at NF34/35 by in situ hybridization for nkx2.1 expression (A, D, G, J, and M) or by for Nkx2.1 (red), Sox2 (blue), and FoxF1 (green) (B, E, H, K, N). At NF42 siblings were analyzed by in situ hybridization for sftpc expression (C, F, I, L, O). P–Y: The expansion of respiratory fate by Wnt/b-catenin requires BMP signaling. Embryos were injected at the 4-cell stage in both dorsal-anterior blastomeres with control-MOs or wnt2/2b-MOs (10 ng/blastomere) and then injected at the 8-cell stage dorsal-vegetally with 800 pg GR-LefdN-BCTA mRNA (400 pg/blastomere). Subsequently the embryos were treated with DMSO, DMH-1, or PD173047 from NF25-35, and then with dex from NF28-35 and analyzed by in situ hybridization for nkx2.1 at NF35 (P,R,T,V,X) and sftpc at NF42 (Q,S,U,W,Y).

Gene Clone Species Stages Anatomy
sftpc.S laevis NF stage 37 and 38 endoderm , foregut , lung primordium , epithelial cell
sftpc.S laevis NF stage 42 endoderm , trachea , lung , foregut , epithelial cell
sftpc.S laevis NF stage 49 endoderm , trachea , lung , foregut , epithelial cell
sftpc.S laevis NF stage 59 endoderm , trachea , lung , foregut , epithelial cell

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  Figure 3. Molecular characterization of the developing Xenopus foregut and respiratory epithelium. A: Schematic of the developing foregut region and respiratory system at the indicated developmental stages, with endoderm in yellow, heart (h) in orange, and respiratory epithelium in blue. B–Y: Whole-mount in situ hybridization with the indicated probes to whole embryos at NF32 (B,F,J), NF33 (N), NF35 (R), NF38 (V) or to isolated guts at NF42 (C,G,K,O,S,W) or to lung/tracheal/esophageal regions dissected at NF49 (D,H,L,P,T,X) or NF59 (E,I,M,Q,U,Y). Abbreviations: e, esophagus; fg, foregut; h, heart; lb, lung bud; lg, lung; lv, liver; st, stomach; tr, trachea; ty, thyroid. Scale bar = 0.5 mmDownload figure to PowerPoint