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Experiment details for sftpc

Rankin SA et al. (2012) Assay

Suppression of Bmp4 signaling by the zinc-finger repressors Osr1 and Osr2 is required for Wnt/β-catenin-mediated lung specification in Xenopus.

Gene Clone Species Stages Anatomy
sftpc.S laevis NF stage 41 lung

  sftpc (surfactant, pulmonary-associated protein C ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 41, ventral view view, anterior up.

Gene Clone Species Stages Anatomy
sftpc tropicalis NF stage 42 endoderm , lung , foregut

  sftpc (surfactant, pulmonary-associated protein C ) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 42, isolated gut tube, anterior left.

Gene Clone Species Stages Anatomy
sftpc.S laevis NF stage 42 lung , lung primordium

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  Fig. 5. Osr1/Osr2 act upstream of Wnt-mediated pulmonary specification and RA-regulated lung bud growth. (A-G) Osr1/Osr2-MO- and MM-MO-injected embryos were treated at stages 25-35 with DMSO vehicle, 10 μM BIO or 1 μM all-trans-retinoic acid (RA), and analyzed for nkx2.1, sox2, wnt2b and raldh2 at stage 35 (A-F) and sftpc at stage 42 (dorsal view) (G). Black arrows indicate normal expression, red arrows show absent expression, green arrows and lines indicate expanded expression and white arrows indicate rescued expression.

Gene Clone Species Stages Anatomy
sftpc.S laevis NF stage 42 lung

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  Fig. 6. Osr1/Osr2 promotes lung specification and growth by restricting BMP signaling. (A). Analysis of Osr1/Osr2-MO and control MM-MO stage 35 embryos indicated that expression of bmp4 and BMP-target genes msx1 and hand1 are expanded into the ‘wnt2b-domain’ of the lpm on the Osr1/Osr2-depleted side. Black arrows indicate normal expression, red arrows indicate absent expression and green arrows indicate enhanced expression. (B) Confocal immunostaining of stage 35 embryos for phospho-Smad1/5/8 (green) and fibronectin (red). Scale bars: 50 μm. White arrows in B indicate ectopic pSMAD1 expression in dorsal foregut endoderm. (C) Analysis of nxk2.1, sox2, wnt2b and raldh2 at stage 35 or sftpc at stage 41 in embryos injected with Osr1/Osr2-MOs or MM-MOs and treated at stages 25-35 with either DMSO (vehicle control) or 10 μM LDN193189. Insets show ventral views of wnt2b expression. For analysis of sftpc expression at stage 41, embryos were removed from the LDN193189 at stage 35 and further cultured in DMSO vehicle until stage 41. Black arrows indicate normal expression, red arrows indicate absent expression, green arrows indicate expanded expression and white arrows indicate rescued expression.