|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
sox2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 42
|
endoderm
,
esophagus
,
stomach
|
nkx2-1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
brain
,
forebrain
,
endoderm
,
lung
,
foregut
,
[+]
|
nkx2-1
|
|
laevis
|
NF stage 42
|
endoderm
,
trachea
,
lung
,
foregut
,
lung primordium
|
nkx2-1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
endoderm
,
respiratory system
,
lung
,
lung primordium
|
|
|
Fig. 2. Osr1/Osr2 are required for respiratory specification and tracheal-esophageal separation. (A-L′) Embryos were co-injected with control MM-MOs or Osr1/Osr2-MOs and GR-mOsr1 RNA (250 pg) in both dorsal-vegetal blastomeres at the eight-cell stage (bilateral injection) and then cultured ±dexamethasone from stage 20 onwards. (A-C,G-I) Analysis by in situ hybridization of nkx2.1 at stage 34/35 (A-C) and sftpc at stage 42 (G-I). (D-F′,J-L′) Confocal immunofluorescence of Nkx2.1 (green) and Sox2 (red) at stage 35/36 (D-F) and stage 42 (J-L). es, esophagus; tr, trachea; lb, lung buds; st, stomach, Scale bars: 100 μm. [Curators Note: all embryos X. laevis] |