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Fig. 1. Expression of sftpc in early lung development of X. laevis. (A) Undetectable sftpc expression at stage 35/36. (B) Earliest expression of sftpc at stage 37/38. (C) sftpc expression is specifically restricted to the lung bud at stage 39. (D, E and F) Cross section of a stage 39 embryo shows sftpc expression in lung buds. Note that the whole lung is located ventral to the esophagus (es). (G) Ventro–lateral view of sftpc expression in lungs at stage 41. (H) Epithelial expression of sftpc in the anterior part of lungs at a point just before the separation of the two lung tubes. (I) Epithelial expression of sftpc in the two separated posterior lung tubes. (I′) The image of the boxed region in (I) shows sftpc staining in the epithelium. Note that at this stage, with the elongation of lungs, the posterior parts of the lung tube extended to the dorsal side of the esophagus. (J) Ventro–lateral view of sftpc expression in lungs at stage 45. sftpc is expressed in the lungs anterior epithelium (K) and posterior epithelium (L). (L′) The image of the boxed region in (L) shows sftpc staining in the epithelium layer. Note at stage 45, the posterior part of lungs is located dorsal to the midgut. Red arrows and dotted red circles indicate lungs. Abbreviations: es, esophagus; mg, midgut; nc, notochord. |