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Figure 4. Expression of XlSALL4 during normal Xenopus limb development. A–I,K,L: Whole-mount in situ hybridization with the anti-sense XlSALL4 probe of stage 48 (A), 50 (B), 51 (C), 52 (D), 52+ (E), 53 (F), 54 (G), 55 (H), 57 (I) hindlimbs and stage 51+ (K), stage 53+ (L) forelimbs. J: A stage 53 hindlimb sense XlSALL4 probe control is shown. Dorsal views with the posterior side (pigmented) up and proximal to the left. Melanocytes are colored brown, while positive XlSALL4 signals are blue/purple. Magnifications are variable to fit different size limbs into constant boxes. |