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Figure 8
Misregulation of CaM KIV does not perturb the specification of hematopoietic fate. (A) Whole-mount in situ hybridization analysis of Xaml expression in neurula stage 18 embryos that had been injected near the VMZ at the four-cell stage with RNAs encoding β-galactosidase (control) or mutant CaM kinases. Ventral views are shown, anterior is to the right. (B) RT-PCR analysis of GATA-1 expression in neurula stage 18 and tailbud stage 28 embryos that had been injected near the VMZ at the four-cell stage with RNAs encoding β-galactosidase (control) or mutant CaM kinases as indicated above each lane. ODC is a loading control. |
