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Experiment details for runx1

Green YS and Vetter ML (2011) Assay

EBF factors drive expression of multiple classes of target genes governing neuronal development.

Gene Clone Species Stages Anatomy
runx1.L runx1-774 laevis NF stage 15 neuron , chordal neural plate border

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  Figure 2. Downregulation of transcription factor candidate target genes after knockdown of EBF2 and EBF3. One cell of two-cell stage embryos was injected with either control MO, both EBF2 MO and EBF3 MO (2MO + 3MO), or dominant negative Xenopus EBF3 (DN-XEBF) mRNA. β-Galactosidase (β-gal) mRNA was co-injected as a marker of the injected side. In all panels the right side is the injected side, showing the light blue color of X-gal staining. (A-C) The expression of sox2 was not changed in all three conditions. (D-O) The expression of nscl-1 (E,F), neurod (H,I), aml1 (K,L), and emx1 (N,O) is downregulated by EBF2 MO and EBF3 MO, and by DN-XEBF (brackets), while control MO does not change their expression levels (D,G,J,M). Panels (A-L) show dorsal views of neurula stage embryos (stage 15/16), and (M-O) are anterior views of tail bud stage embryos (stages 25 to 28).

Gene Clone Species Stages Anatomy
runx1.L runx1-774 laevis NF stage 15 Rohon-Beard neuron , neuron
runx1.L runx1-774 laevis NF stage 23 posterior neural tube , neuron
runx1.L runx1-774 laevis NF stage 28 otic vesicle , ventral blood island , olfactory bulb

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  Figure 4. Neuronal expression for ebf genes and transcription factor candidate target genes. (A-F) ebf2 (A-C) and ebf3 (D-F) are expressed in multiple regions of the developing nervous system, including the trigeminal placodes (yellow arrows), olfactory placodes (black arrows), some domains in the brain, the spinal cord (white arrows), and neural crest derivatives like the branchial arches. (G-L) nscl-1 (G-I) and neurod (J-L) are expressed in the trigeminal placodes and three stripes of primary neurons in the neural plate (black arrowheads) at stage 15, and are strongly expressed in the trigeminal placodes, olfactory placodes, and spinal cord at stage 23. At stage 28, nscl-1 is expressed in the olfactory placodes, some domains in the midbrain/hindbrain, spinal cord and cranial ganglia IX and X. At stage 28, neurod is expressed in the olfactory placodes, retina, otic placodes, cranial ganglia, spinal cord and some domains in the brain. (M-O) aml1 is expressed in the lateral primary neuron stripe at stage 15, sensory neurons of the spinal cord (white arrowheads) at stage 23, and the olfactory placodes and otic placodes at stage 28. (P-R) emx1 is weakly expressed in the prospective forebrain region at stage 15. At stages 23 and 28, this gene is expressed in the dorsal forebrain region. Stage 15 embryos show dorsal views except (P) (anterior view). Stage 23 and 28 embryos show lateral views.