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Figure S4. Control tissues and genes abutting the anterior hemangioblast are
unaffected in Fli1 morphants.
40 ng Fli1 MO was injected into the DMZ at the 4/8 cell stage and embryos were grown
to stage 17. Rohon Beard neuron expression of Runx1 (A,B) and somite expression of
Lmo2 (C,D) was unchanged in Fli1 morphants. MyoD, Nkx2.5, Blimp1 and Bmp4
expression abuts the anterior hemangioblast but in no case does expression of these genes
increase in the hemangioblast territory (asterisks) in Fli1 morphants (E-L), indicating that
in the morphants re-specification is not the reason for lost hemangioblast gene
expression.
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Figure S3. Loss of gene expression in the pVBI and PND in Fli1 morphants.
40 ng Fli1 MO was injected into the VMZ at the 4 cell stage, a region containing the
precursors of the pVBI and PND as well as the DLP. Runx1 and αT4globin (blood genes
not expressed in the DLP) were down regulated in Fli1 morphants (red arrows, A-D)
along with PND markers Pax2 and Pax8 (blue arrows, E-H), although the PND genes
were less sensitive to the MO than the blood genes. Partial down regulation of genes in
the aVBI results from leakage of MO from posterior to anterior at time of injection.
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