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Figure 3. Overexpression of hspa9 enlarges the size of the pronephric tubule. A–I: pax8 whole-mount ISH at early neurula stage following injection of 0, 0.3 or 3.0 ng of hspa9. Dorsal and lateral views show the otic territory (ot) and kidney field (kf) specifically labeled by pax8. Delineations of both areas are depicted. Red-Gal staining indicates the side of injection. J: Quantitative analysis of overexpression of hspa9 on otic and renal territory, expressed as percent of the uninjected side. K,L: At late tail bud stage, pax8 expression seems stronger in the proximal part of the pronephros derived from the injected side, as shown by the Red-Gal staining. M–P: smp30, specific proximal tubular marker, confirms the findings at late tail bud stage (M,N) and tadpole stage (O,P). Q,R: 3G8 immunofluorescence in st32 embryos. S,T: Double-immunofluorescence with 3G8 (black arrows) and 4A6 (red arrows) at st.42 illustrates a longer tubule on the injected (X-Gal stained) than on the noninjected side. U: Histograms represent the summary and the frequency of the enlarged pronephric tubule observed in hspa9-injected embryos, at 0.3 (n = 32) and 3 ng (n = 68).Download figure to PowerPoint |
