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Fig. 8. XPteg regulates pronephric mesoderm formation downstream of RAR signaling. (A) XPteg could restore disrupted expression of SMP30 and Pax2 in embryos expressing a dominant negative retinoic acid receptor α (dnRARα) (white arrowheads). (B) VP16-RARα, a constitutively active form of RARα could not rescue reduced expression of SMP30 and Pax2 caused by XPteg MO (black arrowheads). Eight-cell stage embryos were injected in the presumptive pronephric region with the combination of dnRARα (500 pg), VP16-RARα (500 pg), XPteg (250 pg), XPteg MO (10 ng) as indicated, cultured until stage 36 and subjected to in situ hybridization against SMP30 and Pax2. |
