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Figure 3. Effects of Xhairy2 knockdown on the expression of lens and retinal marker genes in tail-bud-stage embryos. Co MO (3.4 ng: a-f') or Xhairy2 MO (6.9 ng: g-l'), together with EYFP and lacZ mRNA, was micro-injected into one dorsal animal blastomere at the 8-cell stage, and the embryos were harvested at st. 25 (for six6), st. 28 (for notch2, foxE3, L-maf, gamma 1-cry), or st. 32 (for rx1), followed by WISH. MO-receiving cells were visualized by X-gal staining (green). Red arrowheads indicate reduction of marker gene expression. Note that Xhairy2 knockdown reduced the expression of LP marker genes, while virtually no effect was seen on the expression of retinal marker genes six6 and rx1. |
