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Figure 4. The SzlD92N Point Mutation Mimicking Zebrafish ogon Inhibits Xlr Binding and Biological Activity(A) Diagram of Szl-Fc and SzlD92N/Ogon-Fc constructs.(B) BIAcore measurements of binding of 10 μg/ml Xlr in the flow to wild-type Szl-Fc or SzlD92N/Ogon-Fc immobilized on the chip. Note the ten-fold decrease in binding affinity to Xlr.(C–H) Microinjection of 40 nl of 25 μM wild-type Szl-Fc protein into the blastula cavity dorsalizes the Xenopus embryo (E) and rescues the Szl knockdown phenotype (F). Injection of same concentration of SzlD92N/Ogon-Fc protein had no obvious phenotypic effect on wild-type (G) or Szl MO (H) embryos. |
