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Fig. 6. Functional activity of Xhmgb3 in the anterior neural plate. An embryo (stage 13) (A), embryos (stages 17/18) (B–L). Embryos were injected with synthetic RNAs and/or Xhmgb3 MO as indicated at the top of each panel (A–L). Expression of rax (A, B, C, F, G and H), Pax6 (D), Six3 (E), XOptx2 (I and J) or XEn2 (K and L). The injected side is indicated by β-gal activity in blue (B–L) or by a yellow arrow (A). |
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Fig. 4. Rax is required for the initial expression of XOptx2 mRNA in Xenopus. (A–I) Embryos were injected at 4- or 8-cell stage into one dorsal blastomere on the animal side and injected side is indicated by β-gal activity in blue. Embryos (stages 17/18) injected with rax MO or rax-EnR RNA with β-gal RNA (A–H). Expression of XOptx2 (A, B and F), Pax6 (C, D and G) or Six3 (E, H). Anterior views with dorsal to the top (A–H). (I) Embryos (stages 37/38) injected with rax MO (0.5 pmol). Dorsal views with anterior to the top (I). Yellow arrows indicate reduced eyes (I). |