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Fig. 6. miR-200b MO phenotype. A. The injection of miR-200b MO led to abnormal eye structures or even absent eyes on the injected side (white arrows). Histological sections confirmed this observation. A quantitative representation depicts the dose-dependency of this MO effect. B. The expression of Rx1, Pax6 and Sox3 was not affected upon injection of 40 ng miR-200b MO. C. At stage 23, Rx1 and Pax6 expression are downregulated after loss of miR-200b (black arrows). Other examined brain markers were not affected. D. Alcian blue staining at stage 46. MiR-200b deficient embryos showed smaller cranial cartilage structures on the injected side (black arrows). A quantitative representation is given. E. Slug and FoxD3 expression at stage 17 was not affected upon miR-200b depletion. F. The injection of 40 ng miR-200b MO had no effect on the expression of FoxD3 at stage 20 and Krox20 and Twist at stage 23. A quantitative representation is shown. n = number of independent experiments; N = number of analyzed embryos. |
