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Figure 3. Effects of xHtrA1 on Early Pattern FormationWhole-mount in situ hybridization of embryos in lateral (A–D), anterior (E–H), ventral (I–L), and dorsal view (M–T). Embryos were animally injected into one blastomere at the 4-cell stage with nlacZ mRNA as control or xHtrA1 mRNA (each 80 pg).(A–D) In early gastrula embryos, xHtrA1 mRNA blocks expression of Otx2 in the anterior ectoderm and expands Xbra expression into the animal hemisphere.(E–L) At the neurula stage, xHtrA1 mRNA blocks expression of BF1, Rx2a, Krox20, and Nkx2.5. Sizzled expression is reduced anteriorly but significantly expanded at the posterior end of the embryo.(M–T) xHtrA1 mRNA causes expansion of Sox2 and downregulation of Slug and Cytokeratin expression. N-tubulin expression is ectopically induced.Indicated effects on gene expression were observed in B, 45/45; D, 20/23; F, 22/23; H, 16/20 (Rx2a) and 6/20 (Krox20); J, 28/36; L, 31/36; N, 62/67; P, 9/9; R, 19/30; and T, 34/49 embryos. |
