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Fig. 3. B56ε is required for the induction of a subset of EFTFs. (A) Whole mount in situ hybridization showing the expression of rx, lhx2, optx2, six3, and pax6 in stage 14 uninjected embryos (upper panels) and embryos bilaterally injected with 7.5 ng of εmor (lower panels). (B) RT-PCR showing the expression of rx, lhx2, pax6, six3, and ET in control embryos, embryos bilaterally injected with 5 ng or 7.5 ng of εmor from stage 10 (the beginning of gastrulation) to stage 15 (mid-neurula stage). ODC was used as loading control. (C) Whole mount in situ hybridization showing the expression of rx (left column) and lhx2 (right column) in stage 14 uninjected embryos (upper panels), embryos injected with εmor (7.5 ng, middle panels), and embryos injected with εmor (7.5 ng) and ε-c (100 pg) (lower panels). Both dorsal animal blastomeres were injected at the 8-cell stage. Morpholino and ε-c were injected sequentially. Note that the expression of rx and lhx2 was partially rescued by ε-c. |
