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prox1xenopus   

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Experiment details for prox1

Nosip functions during vertebrate eye and cranial cartilage development.

Nosip functions during vertebrate eye and cranial cartilage development.

Gene Clone Species Stages Anatomy
prox1.L laevis NF stage 29 and 30 to NF stage 35 and 36 lens , retina , eye , retinal neural layer

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  Fig. 2: Nosip expression in early embryonic development of Xenopus laevis. A Animal (St. 1), anterior (St. 14, 20 and 23) and lateral (St. 9, 23, 32, 35 and 40) views are shown. Whole mount in situ hybridization (WMISH) experiments show a maternal nosip expression at stage 1 and 9 in the animal pole of the Xenopus embryo (black arrowheads). At stage 14 and 20, nosip transcripts are detected in the anterior neural plate including the eye field (red arrowhead) and the lateral plate border where the neural crest cells are induced (white arrowhead). At stage 23, nosip transcripts are visualized in the eye vesicle (red arrowheads and the migrating neural crest cells (white arrowheads). The dashed line shows the level of the transversal section in a. At stages 30 and 35, nosip is detected in the developing eye (red arrowheads) and the migrating neural crest cells (white arrowheads) of the mandibular (MA), branchial (BA) and hyoidal (HA) arches. B Compared expression of nosip and the two neural crest cell specific marker genes twist and snai2. nosip transcripts are detected in the lateral plate border at stage 14 where anterior neural crest cells are induced (dotted circles). At stage 20, nosip is expressed in the migrating neural crest cells positive for twist and snai2 (white arrowheads). Dotted circles highlight the cement glands. C Nosip expression is visualized in the neural crest of the mandibular (MA), branchial (BA) and hyoidal (HA) arches at stage 35 (horizontal section). At stage 40, nosip is expressed in the gills (middle panel, horizonal section; right panel, transversal section, white arroheads). D Compared expression of nosip and the two eye-specific marker genes prox1 and rax. The upper two rows represent transversal sections, the lower row horizontal sections. At stage 30, nosip is visualized in the lens (le) and the retinal (r) progenitor cells. At stage 35, nosip expression is detected in the lens and the retina with strongest level in the ciliary marginal zone (cmz).

Gene Clone Species Stages Anatomy
prox1.L laevis NF stage 42 retina , eye , retinal neural layer , horizontal cell

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  Fig. 4: Loss of Nosip leads to disorganized retinal layers. Vibratome sections upon Nosip MO injections and WMISH approaches at stage 42. Nosip depletion results in deformed and disorganized retinal layers including rosette like structures (close-up views) shown by the retina cell type specific marker genes rho (photoreceptor cells; 5 of 9 analysed embryos showed a similar phenotype), prox1 (horizontal cells; 4 of 11 analysed embryos), vsx1 (bipolar cells; 8 of 16 analysed embryos), pax6 (amacrine and ganglion cells; 5 of 9 analysed embryos) and pou4f1 (ganglion cells; 9 of 13 analysed embryos). The retinal pigmented epithelium is also affected upon Nosip loss of function (red arrowheads). In contrast, retinal layers of the internal control are unaffected.