|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
pax6
|
|
laevis
|
NF stage 42
|
retina
,
eye
,
retinal ganglion cell layer
,
amacrine cell
,
retinal ganglion cell
|
prox1
|
|
laevis
|
NF stage 42
|
retina
,
eye
,
retinal neural layer
,
horizontal cell
|
vsx1
|
|
laevis
|
NF stage 42
|
retina
,
eye
,
bipolar neuron
|
rho
|
|
laevis
|
NF stage 42
|
retina
,
eye
,
photoreceptor layer
,
eye photoreceptor cell
|
|
|
Fig. 4: Loss of Nosip leads to disorganized retinal layers.
Vibratome sections upon Nosip MO injections and WMISH approaches at stage 42.
Nosip depletion results in deformed and disorganized retinal layers including rosette
like structures (close-up views) shown by the retina cell type specific marker genes
rho (photoreceptor cells; 5 of 9 analysed embryos showed a similar phenotype),
prox1 (horizontal cells; 4 of 11 analysed embryos), vsx1 (bipolar cells; 8 of 16
analysed embryos), pax6 (amacrine and ganglion cells; 5 of 9 analysed embryos)
and pou4f1 (ganglion cells; 9 of 13 analysed embryos). The retinal pigmented
epithelium is also affected upon Nosip loss of function (red arrowheads). In contrast,
retinal layers of the internal control are unaffected. |