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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
pou2f1	xenopus

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Experiment details for pou2f1

Veenstra GJ et al. (1995) Assay

Dynamic and differential Oct-1 expression during early Xenopus embryogenesis: persistence of Oct-1 protein following down-regulation of the RNA.

Gene	Clone	Species	Stages	Anatomy
pou2f1.S		laevis	NF stage 6.5	animal hemisphere
pou2f1.S		laevis	NF stage 17	ectoderm , neuroectoderm , pre-chordal neural plate , neural plate , anterior , [+] posterior
pou2f1.S		laevis	NF stage 24	ectoderm , brain , tail bud , otic placode , optic vesicle , [+] cranial neural crest
pou2f1.S		laevis	NF stage 28	forebrain , telencephalon , diencephalon , midbrain , hindbrain , [+] mandibular crest , hyoid crest , stomodeal-hypophyseal primordium , neuroectoderm , eye , cranial neural crest , anterior branchial crest , posterior branchial crest
pou2f1.S		laevis	NF stage 35 and 36	brain , forebrain , telencephalon , diencephalon , midbrain , [+] hindbrain , eye , central nervous system , chondrocranium
pou2f1.S		laevis	NF stage 41	otic vesicle , forebrain , midbrain , hindbrain , spinal cord , [+] mandibular arch , hyoid arch , branchial arch , cartilage tissue , eye , cranial neural crest , chondrocranium , trigeminal nerve

Fig. 3. Localization of Oct-I transcripts in Xenopus embryos as-established by whole mount in situ hybridization. Unless indicated otherwise, photographs show lateral views of embryos oriented with the anterior side to the right. See Table 1 for a key to the capital-indicated embryonic structures. (A) Animal view of an early blastula stage (stage 6.5) embryo. (B) Stage 17 embryo (neurula stage). (C) Embryo of stage 24. (D) Tailbud stage (stage 28) embryo. (E) Stage 32 embryo (tailbud stage). (F) Tadpole stage (stage 41) embryo. The eye is pigmented; barely any transcripts are detected at this stage. (G) Dorsal view of the stage 28 brain. Compare Fig. 4E. (H) Dorsal view of the stage 36 brain.

Gene	Clone	Species	Stages	Anatomy
pou2f1.S		laevis	NF stage 13	ectoderm , neuroectoderm , mesoderm , dorsal marginal zone
pou2f1.S		laevis	NF stage 17	ectoderm , neuroectoderm , pre-chordal neural plate , mesoderm , neural plate , [+] anterior neural fold , posterior
pou2f1.S		laevis	NF stage 22	ectoderm , stomodeal-hypophyseal primordium , head region , neuroectoderm , mesoderm , [+] tail region , otic placode , cranial neural crest
pou2f1.S		laevis	NF stage 32	brain , forebrain , cranial ganglion , neuroectoderm , central nervous system , [+] otic placode , optic vesicle , cranial neural crest
pou2f1.S		laevis	NF stage 35 and 36	lens , retina , brain , mandibular crest , hyoid crest , [+] branchial crest , cranial ganglion , stomodeal-hypophyseal primordium , eye , central nervous system , otic placode , cranial neural crest
pou2f1.S		laevis	NF stage 43 to NF stage 45	olfactory placode , lens , brain , forebrain , telencephalon , [+] diencephalon , midbrain , hindbrain , cranial ganglion , neuroectoderm , eye , central nervous system , otic placode , cranial neural crest

Fig. 4. Localization of Oct-I (pou2f1) protein in Xenopus embryos as determined by whole mount immunohistochemistry using MAb 12F11 (pou2f1 Ab1). Unless indicated otherwise, all panels represent complete maximum projections of embryos that are oriented with the anterior side to the right. See Table I for a key to the capital-indicated embryonic structures. (A) Lateral view of a gastrula stage embryo (stage 13).Arrow head points to the dorsal blastopore lip. (B) Lateral view of a neurula stage embryo (stage 17). (C) Stage 22 embryo (neural tube stage), lateral view. (D) Dorsal view of stage 32 (tailbud stage) embryo. (E) Embryo of stage 36, dorsal view. Compare Fig. 3G. A specific staining is observed in the lens. (F and H) Projections of, respectively, dorsal and ventral halves of the stage 43 embryo. A complete projection is presented in the Figs. 2C and 7A. Aspecific staining is observed in the lens. The fluorescence observed posteriorly in the embryo represents an artefact. (G) Projection showing a lateral view of the stage 45 brain. Wedge shows the pseudo color settings used.

Gene	Clone	Species	Stages	Anatomy
pou2f1.S		laevis	NF stage 17

pou2f1 (POU class 2 homeobox 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 17, lateral view, dorsal up.

Gene	Clone	Species	Stages	Anatomy
pou2f1.S		laevis	NF stage 17	ectoderm , neuroectoderm , chordal neural plate , neural crest , anterior neural fold , [+] cranial neural crest
pou2f1.S		laevis	NF stage 17	ectoderm , neural crest , neural plate

Fig. 5. Spatial restriction of Oct-I expression in the neural plate. (A) Dorsal view of stage 17 embryo showing transcripts hybridizing laterally in the neural plate (arrow heads). The anterior side of the embryo is oriented to the right. (B) Anterior view of the same embryo showing that the stripes of expression (arrowheads) are connected medially at the level of the eye anlagen. Dorsal side is on the left. (C and D) Transversal sections showing Oct-l RNA and W-1 protein laterally in the posterior neural plate (arrow heads). Lower levels of Oct-I protein are observed medially in the neural plate and in the neural crest and the ectoderm (panel D).

Gene	Clone	Species	Stages	Anatomy
pou2f1.S		laevis	NF stage 24	mesoderm , somite
pou2f1.S		laevis	NF stage 28	otic vesicle , brain , hindbrain , central nervous system
pou2f1.S		laevis	NF stage 43	brain , midbrain , hindbrain , hypophysis , central nervous system , [+] neuron , trigeminal nerve , facial nerve , vestibulocochlear nerve , glossopharyngeal nerve , vagus nerve

Fig. 6. Details of Ott-I expression. (A) Parasagittal optical section of a stage 24 embryo showing the presence of W-l protein in the somites. The anterior side of the embryo is on the right. (B) Transversal section of a stage 28 embryo showing the Ocr-I transcripts being spatially restricted in the hindbrain. Furthermore, Ott-1 RNA is detected in the otic vesicle. (C-F) Frontal optical sections of the stage 43 embryo. The anterior side of the embryo is on the right. (C) Oct-1 protein is detected in the infundibulum (neurohypophysis) and the chiasmatic ridge. (D) Section more ventral in comparison with the section shown in panel C, showing Ott-I protein being present in the adenohypophysis. (E) Spatial restriction of Ocr-I expression in mid- and hindbrain. The highest levels of Dct-I protein are observed in the midbrain at the border with the hindbrain. (F) Maximum projection of frontal sections containing the otic vesicle and the cranial sensory ganglia. Roman numbers refer to the numbers of the cephalic nerves that relay in the Dct-I positive ganglia. The ganglia of the facial nerve (VII) contain relatively low levels of the protein at this stage of development. Note that the other sensory relay centers of the cephalic nerves, i.e., the olfactory bulb and the retina, also contain relatively high levels of Ott-I protein at least at some stage of development (compare Fig. 4D-G).

Gene	Clone	Species	Stages	Anatomy
pou2f1.S		laevis	NF stage 43 to NF stage 44	otic vesicle , brain , forebrain , midbrain , spinal cord , [+] pharynx

Fig. 7. No correlation of &r-I expression with cell density or cell proliferation. (A) Localization of Oct-I protein in the stage 43 embryo as deter- mined by whole mount immunohistochemistry. (B) Spatial differences of cell density in the stage 44 embryo as determined by labeling with TOPRO- 3, a DNA binding dye. (C)Whole mount immunohistochemical staining of a stage 43 embryo performed with MAb PCIO, reactive with Proliferative Cell Nuclear Antigen (PCNA). High levels of PCNA are dt!ected dorso-laterally in the mid- and forebrain.