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pitx2xenopus   

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Experiment details for pitx2

Cast AE et al. (2012) Assay

An essential and highly conserved role for Zic3 in left-right patterning, gastrulation and convergent extension morphogenesis.

Gene Clone Species Stages Anatomy
pitx2.S laevis NF stage 29 and 30 cement gland , heart , lateral plate mesoderm , profundus ganglion , trigeminal ganglion

  Fig. 7. Abnormal L–R molecular marker expression in Xenopus and zebrafish Zic3 morphants. (A) Pitx2 expression in Xenopus Zic3 morphant embryos at stage 30. Embryos were photographed from both left and right sides. Red arrows highlight pitx2 expression. (B) Quantitative results of pitx2 expression in non-injected control (CTRL), control morpholino (CTRL MO) and Zic3 splice site morpholino (SS MO). (C) Southpaw (spaw) expression in zebrafish Zic3 morphant embryos. Red arrows highlight gene expression as left, right, bilateral or absent. (D) Quantitative results of spaw expression in control and Zic3 morphants.