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pibf1xenopus   

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Experiment details for pibf1

The Frog Xenopus as a Model to Study Joubert Syndrome: The Case of a Human Patient With Compound Heterozygous Variants in PI...

The Frog Xenopus as a Model to Study Joubert Syndrome: The Case of a Human Patient With Compound Heterozygous Variants in PIBF1.

Gene Clone Species Stages Anatomy
pibf1.L laevis NF stage 3 (4-cell) animal hemisphere
pibf1.L laevis NF stage 10 involuting marginal zone
pibf1.L laevis NF stage 15 axial mesoderm , paraxial mesoderm
pibf1.L laevis NF stage 20 notochord , somite , multiciliated epidermal cell
pibf1.L laevis NF stage 25 otic vesicle , pronephric mesenchyme
pibf1.L laevis NF stage 29 and 30 brain , spinal cord , head region , notochord , somite , [+]
pibf1.L laevis NF stage 37 and 38 brain , spinal cord , head region
pibf1.L laevis NF stage 45 retina , head region , retinal inner nuclear layer

  Figure 3. Embryonic pibf1 expression correlates with ciliated tissues. Embryos of defined stages were analyzed for pibf1 mRNA by whole-mount in situ hybridization using a digoxigenin-labeled antisense probe. (A) Maternal transcripts in the animal hemisphere of the 4-cell embryo. (B) Expression in the involuting marginal zone tissue of the early gastrula embryo. (C) Staining of pibf1 in the axial and paraxial mesoderm at early neurula stages. (D) Pibf1 signals in skin MCCs and axial/paraxial mesoderm. (E) Expression in the otic vesicle at stage 25. (F–H) Expression pattern in tadpoles, including the head region, somites, and nephrostomes. Histological section of the eye (H′) revealed signals in the retina as well as the inner nuclear cell layer. Planes of histological sections in (B′,D′,E′,F′,H′) are indicated in the respective panels. an, animal; a, anterior; d, dorsal; DL, dorsal lip; INL, inner nuclear layer; l, left; neph, nephrostomes; no, notochord; nt, neural tube; ov, otic vesicle; p, posterior; r, right; re, retina; som, somite; v, ventral; veg, vegetal.

Gene Clone Species Stages Anatomy
pibf1.L laevis NF stage 3 (4-cell) animal hemisphere

  pibf1 (progesterone immunomodulatory binding factor 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 3, horizontal view, animal up.

Gene Clone Species Stages Anatomy
pibf1.L laevis NF stage 29 and 30 basal body , multiciliated epidermal cell

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  Figure 4. Pibf1 localization to MCC basal bodies is attenuated in nonsense JS variant. (A–C) Localization of the endogenous Pibf1 protein, using an anti-PIBF1 antibody (A) and co-staining with Centrin 1 (B) and Hoechst 33342 to highlight the nucleus (C). (D–F) An EGFP-PIBF1 fusion protein recapitulates the staining of the endogenous protein. (G–I) Unaltered expression of the missense allele Y503C. (J–L) Attenuated basal body localization of the truncated Q485* variant of PIBF1. Arrowheads mark protein aggregates observed upon overexpression of EGFP fusion

Gene Clone Species Stages Anatomy
pibf1.L laevis NF stage 29 and 30 basal body , multiciliated epidermal cell

  Figure 5. Loss of Pibf1 protein in pibf1 morphant Xenopus skin MCCs. Basal body staining of Pibf1 (A–C) was lost in TBMO injected specimen (D–F). Tjp1 immunofluorescence was used to mark cell boundaries (B,C,E,F). Fluorescent dextrane (green) was co-injected as lineage tracer to control targeting of injections (E,F). Arrowheads highlight MCC cilia in WT embryo.

Gene Clone Species Stages Anatomy
pibf1.L laevis NF stage 29 and 30 brain , spinal cord , head region , notochord , somite , [+]

  pibf1 (progesterone immunomodulatory binding factor 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 30, lateral view, anterior left, dorsal up.