|
Gene |
Clone |
Species |
Stages |
Anatomy |
pibf1.L
|
|
laevis
|
NF stage 3 (4-cell)
|
animal hemisphere
|
pibf1.L
|
|
laevis
|
NF stage 10
|
involuting marginal zone
|
pibf1.L
|
|
laevis
|
NF stage 15
|
axial mesoderm
,
paraxial mesoderm
|
pibf1.L
|
|
laevis
|
NF stage 20
|
notochord
,
somite
,
multiciliated epidermal cell
|
pibf1.L
|
|
laevis
|
NF stage 25
|
otic vesicle
,
pronephric mesenchyme
|
pibf1.L
|
|
laevis
|
NF stage 29 and 30
|
brain
,
spinal cord
,
head region
,
notochord
,
somite
,
[+]
|
pibf1.L
|
|
laevis
|
NF stage 37 and 38
|
brain
,
spinal cord
,
head region
|
pibf1.L
|
|
laevis
|
NF stage 45
|
retina
,
head region
,
retinal inner nuclear layer
|
|
|
Figure 3. Embryonic pibf1 expression correlates with ciliated tissues. Embryos of defined stages were analyzed for pibf1 mRNA by whole-mount in situ hybridization using a digoxigenin-labeled antisense probe. (A) Maternal transcripts in the animal hemisphere of the 4-cell embryo. (B) Expression in the involuting marginal zone tissue of the early gastrula embryo. (C) Staining of pibf1 in the axial and paraxial mesoderm at early neurula stages. (D) Pibf1 signals in skin MCCs and axial/paraxial mesoderm. (E) Expression in the otic vesicle at stage 25. (F–H) Expression pattern in tadpoles, including the head region, somites, and nephrostomes. Histological section of the eye (H′) revealed signals in the retina as well as the inner nuclear cell layer. Planes of histological sections in (B′,D′,E′,F′,H′) are indicated in the respective panels. an, animal; a, anterior; d, dorsal; DL, dorsal lip; INL, inner nuclear layer; l, left; neph, nephrostomes; no, notochord; nt, neural tube; ov, otic vesicle; p, posterior; r, right; re, retina; som, somite; v, ventral; veg, vegetal. |
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pibf1 (progesterone immunomodulatory binding factor 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 3, horizontal view, animal up. |
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Display additional annotations [+]
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Figure 4. Pibf1 localization to MCC basal bodies is attenuated in nonsense JS variant. (A–C) Localization of the endogenous Pibf1 protein, using an anti-PIBF1 antibody (A) and co-staining with Centrin 1 (B) and Hoechst 33342 to highlight the nucleus (C). (D–F) An EGFP-PIBF1 fusion protein recapitulates the staining of the endogenous protein. (G–I) Unaltered expression of the missense allele Y503C. (J–L) Attenuated basal body localization of the truncated Q485* variant of PIBF1. Arrowheads mark protein aggregates observed upon overexpression of EGFP fusion |
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Figure 5. Loss of Pibf1 protein in pibf1 morphant Xenopus skin MCCs. Basal body staining of Pibf1 (A–C) was lost in TBMO injected specimen (D–F). Tjp1 immunofluorescence was used to mark cell boundaries (B,C,E,F). Fluorescent dextrane (green) was co-injected as lineage tracer to control targeting of injections (E,F). Arrowheads highlight MCC cilia in WT embryo. |
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pibf1 (progesterone immunomodulatory binding factor 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 30, lateral view, anterior left, dorsal up. |