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Figure 2. Expression of the PIAS genes during early Xenopus development. A: Temporal expression analyses by RT-PCR showed that all four PIAS genes were maternally expressed, and their expression persisted until at least tailbud stages. B: Spatial distribution of PIAS transcripts assayed by in situ hybridization revealed that the PIAS genes had overlapping and distinct expression patterns. The embryos are shown at mid-gastrula stages in columns 1 (vegetal view) and 2 (lateral view), late gastrula/early neurula stages in column 3 (lateral view), neurula stages in column 4 (dorsal view), and tailbud/early tadpole stages in column 5 (lateral view). The bisected embryos from tailbud stage embryos are shown in column 6. The labeled structures are: NT, neural tube; not, notochord; NC, neural crest; otic, otic placode; olf, olfactory placode; and eye, eye primordium.Download figure to PowerPoint |
