|
|
| |
Fig. 6. Xpat expression in DE-MO injected embryos. (A) DE-MO injected embryos were analyzed for Xpat expression at the indicated stages. Data are shown as the number of embryos with the indicated expression out of the total number tested. (B, B′) Control and DE-MO injected stage 11 embryos analyzed for Xpat expression showing normal Xpat expression located in the posterior endoderm. (C, C′) Control and DE-MO injected stage 17 embryos showing Xpat stained PGC in the endoderm. (D) Control embryo (stage 24) showing normal PGC distribution and (D′) DE-MO injected embryos with tightly clustered PGCs. (E) Control stage 27 embryo with spaced distribution of Xpat-labeled PGCs and (E′) in the DE-MO injected embryo PGCs appear slightly clustered. |
|
Display additional annotations [+]
|
| |
Fig. 5. XDead end protein is important for primordial germ cell development. (A) In the coupled in vitro transcription/translation system (Promega), XDead end and zDead end respectively were translated with or without different amounts of XDead end specific morpholino antisense oligonucleotide (DE-MO) or with control morpholino as indicated. Two-cell stage albino embryos were injected vegetally into both blastomeres with XDead end specific morpholino antisense oligonucleotide (DE-MO) or control morpholino (CO-MO). Stage 31/32 embryos were fixed and analyzed by whole mount in situ hybridization using a digoxigenin labeled antisense RNA probe for Xpat. Visible Xpat positive primordial germ cells (PGCs) were counted. (B) An uninjected stage 31 embryo showing an average number of Xpat expressing primordial germ cells. (C) Example of a stage 31 embryo injected with DE-MO resulting in a reduced number of Xpat containing primordial germ cells. (D) Example of a DE-MO injected stage 31 embryo with a total loss of Xpat positive primordial germ cells. (E) Percentage of embryos with a normal number of PGC marker positive cells (light blue), less or at least 4 PGC marker positive cells (blue) and no PGC marker expressing cells (dark blue) after DE-MO or control MO injection. Usage of Xpat or Xdazl respectively is indicated above the chart. Amount of injected DE-MO or control MO are indicated below the chart. (F) Partial rescue of the DE-MO effect by coinjection of increasing amounts of zebrafish Dead end-globinUTR mRNA. |
