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Fig. 3. Expression of fatvg mRNA in stage 40 tadpoles. (A) fatvg expression was detected at the dorso-anterior margin of the endodermal mass by in situ hybridization, as shown on a transverse section of a tadpole stage embryo. (B) A higher magnification of the boxed area in (A) is shown. (C) fatvg mRNA was detected as two symmetric stripes located on the endodermal mass, as shown on a cross-section of a tadpole along the dorsal-ventral axis at the position indicated in (A). (D) A sense control section of a tadpole at a corresponding position along the dorsal-ventral axis. No staining was detected. (E) Xpat was used as a marker for primordial germ cells. The expression pattern of Xpat is distinct from cells expressing fatvg shown in (C). Arrows indicate cells expressing fatvg mRNA. Arrowhead points to Xpat expressing cells. e, eye; en, endoderm; no, notochord; s, somite. |