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Figure 1: A and B. Representative images of pgat in situ hybridization showing pgat-positive PGCs (blue dots) in control and phthalate mixture-treated Xenopus embryos at NF stage 33 & 34. Embryos were treated with phthalate mixture (100 mg/ml) from NF stage 9 (A) and NF stage 14 (B), respectively. C and D. Quantitation of the primordial germ cells in each treatment group. Primordial germ cells were counted from each embryo in each treatment group. The graph shows the abundance of primordial germ cells in embryos exposed to various doses of phthalate mixture from NF stage 9 (C) and NF stage 14 (D). In order to assess the significance of the data, the unpaired t-test with Welch’s correction was used. *** p<0.005. These experiments were performed three times with similar results. |
