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Experiment details for pgat

Combined functions of two RRMs in Dead-end1 mimic helicase activity to promote nanos1 translation in the germline.

Combined functions of two RRMs in Dead-end1 mimic helicase activity to promote nanos1 translation in the germline.

Gene Clone Species Stages Anatomy
pgat.L laevis NF stage 29 and 30 primordial germ cell

  Figure 2. Overexpression of xDnd1ter or Dnd1 ∆RRM2-R results in loss of PGCs. A-C. One cell embryos were injected vegetally with 500pg of GFP (control) or the indicated dnd1 mRNA. Tailbud embryos (stage 32-35) were analyzed for Xpat expression by WISH. Representative images are shown. D. PGC number per embryo was quantified. GFP, n=16; Dnd1∆RRM2-R, n=32; Dndter, n=16. *P<0.05, compared with GFP control. Analysis based on at least two independent experiments and shown as a box and whisker plot.