|
|
|
Fig. 4.
Abnormal germ plasm aggregation in sybu-depleted embryos. (A-D) Representative embryos at the 4-cell stage, probed for pgat to identify germ plasm, vegetal views. (A) uninjected control embryo, (B) sybu-depleted embryo, using as234, (C) sybu-depleted embryo using as6, (D) sybu-depleted embryo (as234) rescued by injection of sybu mRNA. (A’-D’) Representative embryos similarly analyzed at the 32-cell stage. |
|
|
|
Fig. 3.
Reduction of PGCs in sybu-depleted embryos. (A-C) Whole-mount in situ hybridization for pgat mRNA. (A) control uninjected embryo, (B) sybu-depleted embryo, (C) depleted embryo rescued with injected sybu mRNA (5 pg). (D) Scatter plots of PGC numbers in experimental groups corresponding to the adjacent panel. Dots represent individual in which PGCs were counted (sum of three separate host-transfer experiments); dark line represents the median; shaded areas correspond to the limits of the first and third quartiles (25th/75th percentiles) and whiskers (dotted line) extends out to 1.5x the interquartile range. P<0.001 by Kruskal-Wallis test; *** = P<1.0e-11 by Dunn's post hoc test w/ 2-sided Bonferroni adjustment. |
|
Display additional annotations [+]
|
|
Fig. 1.
Germ plasm enrichment of Sybu mRNA and protein in oocytes and early embryos. (A-D) Representative images of whole-mount in situ hybridization on stage VI oocytes comparing expression of (A) trim36, (B) wnt11b, (C) pgat and (D) sybu. Vegetal views are shown. (E-J) Localization of Sybu-GFP to germ plasm. (E-G) Overlap of Sybu-GFP with clustered mitochondria (mito., MitoTracker) in pricked eggs 60-min post-activation (p.a.) and at 240-min post-activation (p.a.). Scale bars represent 7.5 µm. |