| |
Fig. 3.
WISH of vegetal pole transcripts. The expression of a subset of vegetally enriched mRNAs was analyzed during oogenesis and embryo development by WISH. Expression patterns are grouped according to mRNA pathway: early (A), late (B) or both (C). xpat expression marks germ plasm. Probes, developmental stages and developmental structures are indicated in the illustrations at the top: germ plasm/PGCs (purple), pronephros (pink), ventral blood islands (lime green), eye (black), lens (white), otic vesicle (gray), cranial ganglia (yellow), brachial arches (green), nasal placodes (teal), intersegmental region (brown), notochord (orange), brain and neural tube (blue). Transcripts detected in PGCs at the tailbud stage are shown at higher magnification in insets. WISH analysis was performed on ≥40 total embryos from at least two adult female frogs. Dorsal (D), ventral (V), left (L), right (R), posterior (P), anterior (A), vegetal view (v); MC, mitochondrial cloud; N, nucleus; Oo, oocyte. Scale bars: 100 μm for stage I oocyte; 200 μm in remaining panels. |
