| |
Fig. 4 Overexpression of wild-type (WT) and mutant Germes affects
germ plasm morphology. Embryos from all experimental
groups (A, intact; B, water-injected; C, GermesDEFh mRNA-injected;
D, GermesDLZs mRNA-injected; E, WT Germes mRNAinjected)
were immunostained for Xpat protein (A0–E0). Nuclei
were revealed by DAPI-staining; this, to a lower extent, also stains
yolk platelets (A–E). Merged images (A00–E00) demonstrate exclusively
nuclear Xpat localization in control (A00B00) and GermesDEFh-
expressing (C00) embryos. In embryos injected with
GermesDLZs (D00) or WT Germes (E00) mRNAs, Xpat is also present
in yolk-free cytoplasm around the primordial germ cell (PGC)
nucleus. Arrows point to nuclei. Scale bar516 mm. |
