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Figure 5. Cellular distribution of Hermes/Rbpms, hnRNP I, Vg1RBP/Vera, and Xpat proteins in the stage I/II oocytes. Confocal images showing immunofluorescence (IF) of stage I/II oocyte with (A) anti-Hermes/Rbpms (green) and (A’) anti-Xpat (red) antibodies; (A’’) Superimposed image. White arrows indicate Xpat and Hermes/Rbpms particles are not identical; (B) Superimposed images of hnRNP I (green) and GRP94 (red) proteins. Co-staining with anti-GRP94 reveals the ER enriched within the MC, cortex, and perinuclear region. Note that hnRNP I is excluded from the MC but is present in the nucleus and cytoplasm; (C) Merged images of endogenous Vg1RBP/Vera (red); or (D) hnRNP I (red) with Alexa 488-labeled nanos 3′UTR injected 20 h before fixation (green). Note that while nanos RNA localizes to the MC, both Vg1RB/Vera and hnRNP I are excluded from it. N: nucleus; MC: mitochondrial cloud. Scale bars are as indicated. |
