|
Fig. 3.
Positioning of PGCs along the A/P axis in control and XGRIP2.1 morphant embryos. (A–H) Representative examples of uninjected control embryos exhibiting normal PGC positioning between stage 24 and stage 32. PGCs were stained for Xpat and somites for XMyoD by WMISH. (A–G) Embryos were cleared with benzyl benzoate/benzyl alcohol. (H) PGC migration profile of uninjected control embryos (stage 24–33). (J) The PGC distribution profile of embryos at stage 32 injected vegetally into both blastomeres at the 2-cell stage with 0.2 pmol of MO2 XGRIP2.1 or CO MO. Average PGC numbers at each somite position were calculated from results of 3 independent experiments. A total of 60 embryos were analyzed for each stage of development or treatment.
|