| |
Fig.3. Semithin sections through oocytes hybridized with Xcat2
(A,C) and Xpat (B) antisense RNA probes, labeled with nanogold and
silver enhanced. (A). In a stage II oocyte, the unfragmented mitochondrial
cloud (mc) extends between the layer of homogenous cytoplasm
(stars) surrounding the nucleus (on) and vegetal cortex. The germinal
granules containing localized Xcat2 mRNA (artificially colored in red) are
visible as a layer at the vegetal cortex in the METRO region. (B) Fragment
of stage II oocyte showing the unfragmented mitochondrial cloud with a
layer of Xpat mRNA (outlined artificially in red) at the vegetal cortex in the
METRO region. (C) In a late stage III oocyte, germ plasm islands (fragments
of mitochondrial cloud) are at the vegetal cortex. Arrows indicate
the position of several of germ plasm islands, which are barely visible at
such a low magnification. Bars in A,B, 25 μm; bar in C, 50 μm. |
