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Fig. 3. Localisation of Xpat mRNA assessed by in situ hybridisation. (A) Stage III oocytes (Dumont, 1972); Xpat transcripts are localised to the vegetal pole from stage I (A), remaining there during stages II (B,C) and III (D). (E,F) In cleavage stage embryos the signal takes on a granular appearance in a small disc at the vegetal pole. During gastrulation (stage 10.5, G) the cells that contain Xpat move inside, and remain there through neurulation (stage 14, H) until tailbud (stage 26, I). At stage 35 (J) they have begun to move anteriorly and dorsally in the endodermal mass. This continues through stage 37 (K), until they are most dorsal by stage 40 (L). (G) Embryos are cleared. Scale bar=100 μm. |
