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Fig.7. Nanos1 protein expression during X. laevis development. Endogenous Nanos1 was detected by confocal immunofluorescence with anti-Nanos1 antibody (red in all images). (A) Nanos1 was not expressed in stage VI oocytes. (C) Nanos1 protein was concentrated in a sub-cellular cytoplasmic domain close to the cell periphery in stage 8 embryos. (B, E, G, I) Nanos1 protein co-localizes with the germ plasm at stages 4 (8 cell), 10.5, 12, and 13. Xiwi, a germ plasm specific protein, was detected by anti-Xiwi antibody (green). (D, F, H, J, K) Hyperacetylated histone H4 (Penta), a nuclear marker, was detected by immunostaining (Green). Nanos1 protein (red) was found only in perinuclear locations in stages 10.5, 12, 13, 14, and 28. (L and M) Stages 33/34 and 37/38. Xpat RNA was detected by whole mount in situ hybridization using fluorescein tyramide (green). L′ and M′, same sample as in L and M. Nanos1 protein could no longer be detected in PGCs after stage 34. C–K, images were from the endoderm mass. L and M, lateral view, with anterior to the left. Scale bars are as indicated. |
