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pgatxenopus   

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Experiment details for pgat

Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA.

Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA.

Gene Clone Species Stages Anatomy
pgat.L laevis NF stage 29 and 30 germ plasm , primordial germ cell

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  Figure 4. Expression and germ plasm localization of gptl1. In situ hybridization against gptl1. A: Early stage oocytes (stage I-II, left panel; stage III-IV, right panel). Roman numerals indicate oocyte stages; mc, mitochondrial cloud. B: Stage VI oocyte, vegetal view. C: Four-cell embryo, vegetal view. D: Stage 10.5, vegetal view. E: Stage 30 embryo, cleared in Murray's clear, showing expression in branchial arches and intersomitic boundaries and the absence of staining in primordial germ cells (PGCs). Inset: in situ for xpat showing positive PGC staining in a cleared embryo.